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Macherey-Nagel™ Nucleospin™ 96 Plasmid Transfection-grade
A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Full and Core kits utilize a 96-well plate format for an efficient high-throughput process.
Brand: Macherey-Nagel™ 740492.4
This item is not returnable.
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Description
Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.
- Purifies high-copy plasmids for transfection
- Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
- Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
- Ultra-fast procedure simplifies the work-flow
- High-throughput — 96-well plates allow for easy automation and supports plasmid screening
- Downstream applications: transfection of common cell lines (lipofection, electroporation)
- Also suitable for standard molecular biology application, such as cloning and sequencing
Specifications
| <50 EU/ng DNA | |
| 96-well Plate | |
| Bacterial Culture (E. coli, High-copy Plasmids) | |
| Elution Volume: 100 - 200 μL | |
| Centrifuge, Vacuum manifold (e.g. NucleoVac 96) | |
| Filter Plates, Binding Plates, Buffers, RNase A | |
| Core Kit | |
| Up to 5 mL | |
| 45 min/plate |
| Room temperature (18 - 25 °C) | |
| Silica Membrane and Endotoxin-reduction Technology | |
| Binding capacity: 20 μg | |
| Transfection-grade plasmid DNA | |
| Rapid purification of plasmids for transfection | |
| 4 x 96 | |
| A260/A280: 1.80 - 1.85 | |
| < 15 kbp | |
| 5 - 20 μg |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- NucleoSpin 96 Plasmid T.-gr. Core (4x96)
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H336-May cause drowsiness or dizziness.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P330-Rinse mouth.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
Supplemental information
- MIXTURE LIST-Contains : sodium hydroxide, dodecyl sulphate, guanidine hydrochloride,triethanolamine, 2-propanol, RNAse