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Macherey-Nagel™ NucleoSpin™ Microbial DNA
Kit for reliable isolation of DNA from microorganisms (e.g. yeast, fungi, and gram-negative and gram-positive bacteria), with lysis that is supported by mechanical disruption with glass beads (tubes included).
Brand: Macherey-Nagel™ 740235.50
This item is not returnable.
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Description
The NucleoSpin™ Microbial DNA kit is designed for convenient, fast, and easy isolation of genomic DNA from microbial samples. Because microbial samples can be difficult to lyse (due to their strong complex cell wall structures), the kit replaces enzymatic lysis with mechanical disruption of cell wall structures utilizing NucleoSpin™ Bead Tubes.
- Suitable for a large variety of starting materials
- High yields of genomic DNA can be isolated
- NucleoSpin Bead Tubes Type B included – for efficient lysis, compatible with many disruption devices
- Fast and easy procedure – Liquid Proteinase K included, no additional enzyme required
- Typical downstream applications: PCR, real-time PCR, southern blotting, enzymatic reactions
Specifications
| Mini Spin Column | |
| Bacteria (Gram +/-), Fungi, Yeast | |
| Elution volume: 100 - 200 μL | |
| Centrifuge, Vortex mixer | |
| NucleoSpin Microbial DNA Columns, NucleoSpin Bead Tubes Type B, Collection Tubes (2 mL), buffers, Liquid Proteinase K | |
| A260/A280: 1.6 - 2.0 | |
| 35 min/prep |
| Silica MembraneTechnology and Mechanical (Bead Tube) Disruption | |
| Binding capacity: 60 μg | |
| Genomic DNA | |
| DNA isolation from microorganisms | |
| 50 | |
| < 40 mg wet weight cell pellet | |
| 5 - 25 μg |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- NucleoSpin Microbial DNA
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Specific target organ toxicity Category 3
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H319-Causes serious eye irritation.
- H336-May cause drowsiness or dizziness.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash with water thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P330-Rinse mouth.
Supplemental information
- MIXTURE LIST-Contains : guanidine hydrochloride; 2-propanol; proteinase K, liquid (origin: tritirachiumalbum); guanidinium