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Macherey-Nagel™ NucleoSpin™ Microbial DNA

Kit for reliable isolation of DNA from microorganisms (e.g. yeast, fungi, and gram-negative and gram-positive bacteria), with lysis that is supported by mechanical disruption with glass beads (tubes included).

51.70€ - 1025.00€

Specifications

Format Mini Spin Column
Isolation Technology Silica MembraneTechnology and Mechanical (Bead Tube) Disruption
Sample Type Bacteria (Gram +/-), Fungi, Yeast
Efficiency Binding capacity: 60 μg
Elution Volume Elution volume: 100 - 200 μL
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Products 3
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
15353609
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Macherey-Nagel™
740235.10
10
51.70€
10 reactions
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15373609
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Macherey-Nagel™
740235.50
50
249.00€
50 reactions
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15878096
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Macherey-Nagel™
740235.250
250
1025.00€
250 reactions
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Description

Description

The NucleoSpin™ Microbial DNA kit is designed for convenient, fast, and easy isolation of genomic DNA from microbial samples. Because microbial samples can be difficult to lyse (due to their strong complex cell wall structures), the kit replaces enzymatic lysis with mechanical disruption of cell wall structures utilizing NucleoSpin™ Bead Tubes.

  • Suitable for a large variety of starting materials
  • High yields of genomic DNA can be isolated
  • NucleoSpin Bead Tubes Type B included – for efficient lysis, compatible with many disruption devices
  • Fast and easy procedure – Liquid Proteinase K included, no additional enzyme required
  • Typical downstream applications: PCR, real-time PCR, southern blotting, enzymatic reactions
Specifications

Specifications

Mini Spin Column
Bacteria (Gram +/-), Fungi, Yeast
Elution volume: 100 - 200 μL
Centrifuge, Vortex mixer
NucleoSpin Microbial DNA Columns, NucleoSpin Bead Tubes Type B, Collection Tubes (2 mL), buffers, Liquid Proteinase K
< 40 mg wet weight cell pellet
5 - 25 μg
Silica MembraneTechnology and Mechanical (Bead Tube) Disruption
Binding capacity: 60 μg
Genomic DNA
DNA isolation from microorganisms
A260/A280: 1.6 - 2.0
35 min/prep
Videos
Safety and Handling

Safety and Handling

Product Identifier
  • NucleoSpin Microbial DNA

Signal Word
  • Warning

Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Specific target organ toxicity Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H319-Causes serious eye irritation.
  • H336-May cause drowsiness or dizziness.
  • H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash with water thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.

Supplemental information
  • MIXTURE LIST-Contains : guanidine hydrochloride; 2-propanol; proteinase K, liquid (origin: tritirachiumalbum); guanidinium

SDS
Documents

Documents

Product Certifications