Macherey-Nagel™ NucleoSpin™ Total RNA FFPE XS Column

Description

NucleoSpin FFPE RNA, no restriction for total RNA isolation - even from FFPE samples.
Enhance your RT-PCR* performance. Improved template quality - get less fragmented, well decrosslinked, DNA-free RNA.
Decrosslinking buffer and rDNase provided with the kit. Improved sensitivity - highly concentrated RNA by elution in only 5μL to 30μL. Use of the XS (mini elution volume) column design.
Convenient paraffin removal
Simply melt paraffin with the Paraffin Dissolver (patent pending) provided with the kit. Without toxic organic solvent - without time-consuming mixing steps - without long incubation time.
Save time
Minimum number of working steps. Fast procedure preserves RNA quality.
Suitable for a large variety of starting materials, like clinical samples
e.g., colon carcinoma (hematoxylin stained/unstained), colon mucosa, adenocarcinoma of colon transversum, liver (rat/human), liver carcinoma (hematoxylin stained), lymph node, spleen (rat/human); pancreas (rat/human; diabetic/nondiabetic), placenta.

• Silica membrane technology
• Format: mini spin columns - XS design for increased sensitivity
• Sample material: up to 7 sections of 250mm&superscript_2;, or 15mg
• RNA yield depends on the amount and quality of the sample
• Typical ration A&subscript_begin;260&subscript_end;/A&subscript_begin;280&subscript_end;: 1.9 to 2. 1
• Typical RIN: 2 to 6
• Typical RNA size: 100nt to 5, 00nt
• Elution volume: 5μL to 30μL
• Preparation time: 70min
• Easy paraffin removal
• Convenient handling
• Fast procedure RNA purification from FFPE samples.