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Invitrogen™ PGFM Enzyme Competitive ELISA Kit
Competitive ELISA
Brand: Invitrogen™ EIAPGFMX10
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.
Description
The PGFM Enzyme ELISA quantitates PGFM in serum,plasma,urine,dried fecal extracts,or cell culture medium.
Principle of the method
The PGFM Competitive ELISA research-use-only kit designed to quantitatively measure PGFM present in fecal extracts,urine,serum,and plasma samples. A PGFM standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit IgG. A PGFM-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody highly specific to PGFM to each well. After a 1-hour incubation,the plate is washed and substrate is added. The substrate reacts with the bound PGFM-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
In many species, uterine and placental Prostaglandin F2alpha (PGFM) is involved in the regulation of reproductive and pregnancy-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. Prostaglandin F2alpha is metabolized to PGFM during the first passage through the lungs. PGFM has a longer half-life in peripheral circulation than PGF2alpha and has been applied as a useful analytical marker of PGF2alpha. PGFM is identical across all species and so this kit should measure PGFM from sources other than those tested.
Specifications
| 50 to 3200 pg/mL | |
| PGFM Competitive ELISA Kit | |
| HRP | |
| ELISA Kit | |
| 10 x 96 Assays | |
| ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti | |
| 9.7% | |
| Pre-coated 96-well plate, Standard, Assay Diluent Concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| RUO | |
| Fecal Extract (50 μL), Plasma (6.25 μL), Serum (6.25 μL), Supernatant (50 μL), Urine (6.25 μL) | |
| Chemical | |
| 1 hr. 30 min. |
| 20.8 pg/mL | |
| Multiplex Assay on the Luminex Platform | |
| Fluorescence | |
| Fecal Extract, Plasma, Serum, Supernatant, Urine | |
| Human | |
| Colorimetric Microplate Reader | |
| 9.2% | |
| HRP | |
| Reproductive Biology | |
| -20°C | |
| 1 hr. 20 min. |
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