Invitrogen™ PGFM Enzyme Competitive ELISA Kit

Description

The PGFM Enzyme ELISA quantitates PGFM in serum,plasma,urine,dried fecal extracts,or cell culture medium.

Principle of the method

The PGFM Competitive ELISA research-use-only kit designed to quantitatively measure PGFM present in fecal extracts,urine,serum,and plasma samples. A PGFM standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit IgG. A PGFM-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody highly specific to PGFM to each well. After a 1-hour incubation,the plate is washed and substrate is added. The substrate reacts with the bound PGFM-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

In many species, uterine and placental Prostaglandin F2alpha (PGFM) is involved in the regulation of reproductive and pregnancy-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. Prostaglandin F2alpha is metabolized to PGFM during the first passage through the lungs. PGFM has a longer half-life in peripheral circulation than PGF2alpha and has been applied as a useful analytical marker of PGF2alpha. PGFM is identical across all species and so this kit should measure PGFM from sources other than those tested.