Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Description

Thermo Scientific Phire Hot Start II PCR Master Mix is a convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides, and optimized reaction buffer, including MgCl₂. Only template and primers need to be added to the PCR reaction.

Phire Green Hot Start II PCR Master Mix also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.

Phire Hot Start II DNA Polymerase, included in the master mix, is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain that allows short extension times (10–15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase (Cat. No. EP0072).

Features

• No reactivation step with quick hot start
• Amplify four times faster than with hot start Taq
• Minimal reaction optimization due to high inhibitor tolerance
• Abundant products due to high efficiency
• Amplify significantly longer DNA fragments than with any hot start Taq
• Direct loading on gel with Green format

Applications

• Hot Start PCR
• Routine PCR
• Non-high-fidelity PCR
• Fast PCR
• High throughput PCR
• Genotyping

Notes

• The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.