Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.
Brand: Thermo Scientific™ 26156
Thermo Scientific™ Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.
This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.Highlights:
Includes:
Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls
| >50 mg Human IgG/mL resin binding capacity | |
| Pierce Agarose ChIP Kit | |
| Pierce™ | |
| ChIP Kit | |
| Kit |
| ≥50 mg Human IgG per mL Resin Binding | |
| Sufficient For: 30 complete chromatin IP assay experiments• ChIP Grade Protein A/G Plus Agarose, 0.65 mL (store at 4°C) • IP Dilution/Wash Buffer (5X), 11 mL (store at 4°C) • IP Wash Buffer 3 (5X), 4.5 mL (store at 4°C) • IP Elution Buffer (2X), 4.5 mL (store at 4°C) • Column Accessory Pack, 3 packs (store at room temperature or 4°C) • Microcentrifuge Tubes (1.5mL), 75 tubes (store at room temperature or 4°C) • DNA Clean-Up Column, 40 columns (store at room temperature or 4°C) • DNA Column Binding Solution, 30 mL (store at room temperature or 4°C) • DNA Column Wash Solution, 6 mL (store at room temperature or 4°C) • pH Indicator, 0.8 mL (store at room temperature or 4°C) • DNA Column Elution Solution, 5 mL (store at room temperature or 4°C) • Anti-RNA Polymerase II Antibody, 25 μL (store at -20°C) • Normal Rabbit IgG (1 mg/mL), 10 μL (store at -20°C) • ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL (store at -20°C) • Pierce Chromatin Prep Module (Part No. 26158) (store Proteinase K and Micrococcal Nuclease at -20°C. Store all other components at 4°C). |
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| 30 Reaction Kit | |
| 30 Reactions |
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A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| ZJ398051 | Certificate of Analysis | 16/12/2024 | 26156 |
| ZJ398051A | Certificate of Analysis | 16/12/2024 | 26156 |
| ZH396110 | Certificate of Analysis | 12/09/2024 | 26156 |
| ZH396110A | Certificate of Analysis | 12/09/2024 | 26156 |
| ZH395821A | Certificate of Analysis | 27/08/2024 | 26156 |
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For Research Use Only. Not for use in diagnostic procedures.
