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Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Brand: Thermo Scientific™ 32132X3
733.28 EUR valid until 2024-11-29
Use promo code "21683" to get your promotional price.
This item is not returnable.
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Description
Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.Features of ECL Plus Substrate:
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
Specifications
Substrate | |
Liquid | |
Nitrocellulose, PVDF | |
300 mL Kit | |
3000 sq. cm of membrane |
Chemiluminescence, Fluorescence | |
Western Blot | |
Pierce™ | |
Low picogram to mid-femtogram | |
Upon receipt store at 4°C. Product shipped on ice. |
For Research Use Only. Not for use in diagnostic procedures.