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Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
153.00€ - 838.00€
Specifications
| Product Type | Substrate |
|---|---|
| Detection Method | Chemiluminescence, Fluorescence |
| Form | Liquid |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Product Line | Pierce™ |
| Product Code | Brand | Quantity | Sufficient For | Price | Quantity & Availability | ||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Sufficient For | Price | Quantity & Availability | ||||
|
11547910
|
Thermo Scientific™
32134 |
25 mL Kit | 250 cm2 of membrane |
153.00€
25mL |
In Stock
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|
11527271
|
Thermo Scientific™
32132X3 |
300 mL Kit | 3000 cm2 of membrane |
838.00€
Pack of 3 |
In Stock
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|
11517271
|
Thermo Scientific™
32132 |
100 mL Kit | 10 mini-gel blots, 1000 cm2 of membrane |
350.00€
100mL |
In Stock
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||||
Description
Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.
Features of ECL Plus Substrate:
- Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
- Higher sensitivity—detect targets down to the low-picogram level
- Longer signal duration—sustained light output for as long as 5 hours
- More imaging options—X-ray, CCD or laser-based imagers
- More affordable—high quality and performance at a lower price than other suppliers' similar substrates
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
Specifications
| Substrate | |
| Liquid | |
| Pierce™ |
| Chemiluminescence, Fluorescence | |
| Nitrocellulose, PVDF | |
| Low picogram to mid-femtogram |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Pierce ECL Plus Western Blotting Substrate
Signal Word
- Danger
Hazard Category
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- H351-Suspected of causing cancer.
Precautionary Statement
- P201-Obtain special instructions before use.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Ethanol
For Research Use Only. Not for use in diagnostic procedures.
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Product Title
Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate