Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.
Brand: Thermo Scientific™ 21516
Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.
Features of GST Tag Protein Interaction Pull-Down Kit:
Applications:
You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.
Related Products
Pierce™ His Protein Interaction Pull-Down Kit (Catalog #21277)
| 750 μL | |
| Slurry: 50 % | |
| Affinity Purification | |
| Pierce™ | |
| Pull-Down (Tag-Based) | |
| Pull-Down Kit | |
| Kit |
| 1.5 mL | |
| Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin • Glutathione Agarose, 750 μL • Pull-Down Lysis Buffer, 250 mL • Glutathione, 1 g • TBS Pack (makes 500 mL), 1 pack • Pierce Spin Columns and Accessories, 27 columns • Microcentrifuge Collection Tubes, 100 tubes Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C. |
|
| Microcentrifuge | |
| 25 reactions kit | |
| Not Target-Specific | |
| 25 Reactions |
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For Research Use Only. Not for use in diagnostic procedures.
