missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ Micrococcal Nuclease Solution (≥100 U/μL)
Remove nucleic acids from cell lysates, release chromatin-bound proteins and shear chromatin for chromatin immunoprecipitation (ChIP) using micrococcal nuclease.
Brand: Thermo Scientific™ 88216
133.48 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
This item is not returnable.
View return policy
Description
Thermo Scientific™ Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
This micrococcal nuclease is a stable liquid form of the enzyme derived from Staphylococcus aureus. Micrococcal nuclease (MNase) exhibits exo- and endo-5'-phosphodiesterase activities against DNA and RNA. This enzyme digests double-stranded, single-stranded, circular and linear nucleic acids. The highest activity is toward single-stranded nucleic acid substrates with preference for AT- or AU-rich regions. Enzymatic activity occurs at pH 7 to 10 and is strictly dependent on calcium for the digestion of RNA and DNA substrates. Micrococcal nuclease is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.Highlights:
- Digests nucleic acids (DNA and RNA)
- Effective for double-stranded, single-stranded, circular and linear nucleic acids
- Active in neutral to alkaline buffer conditions containing divalent calcium ions
- Supplied at ≥100 units/μL in 10mM Tris-HCl, pH 7.5, 50mM NaCl, 1mM EDTA, 50% glycerol
Compatible with:
Thermo Scientific Subcellular Protein Fractionation Kit (Part No. 78840); Thermo Scientific Pierce ChIP Kit (Part No. 26156)
Recommended for:
Enzymatic DNA shearing for chromatin immunoprecipitation assays (ChIP); Removal of unwanted genomic or organellar nucleic acids from cell lysates; Degradation of nucleic acids in subcellular fractionation procedures
Specifications
| Cell Lysis Enzyme | |
| Liquid | |
| 150 μL |
| Upon receipt store at -20°C. Product is shipped with dry ice. | |
| Enzyme for Cell Lysis |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction