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Thermo Scientific™ Pierce™ p-Aminophenyl Phosphoryl Choline Agarose
Bind and purify C-reactive protein (CRP) using these agarose beads of immobilized p-aminophenyl phosphoryl choline (APC).
Brand: Thermo Scientific™ 20307
Description
Thermo Scientific™ Pierce p-Amino Phosphoryl Choline Agarose enables the purification of C-reactive protein (CRP).
Immobilized phosphoryl choline is a beaded agarose affinity resin for purification of C-reactive protein (CRP) from plasma, ascites and other biological fluids. CRP is a pentameric (pentraxin) protein that is classified as an acute phase reactant because of its high level of production during inflammation. The protein binds to phosphoryl choline groups on the surface of microbes and has been linked to several biological functions including activation of the classical complement pathway, enhancement of phagocytosis and interaction with certain subpopulations of T-lymphocytes. In the late 1970s, Volanakis and colleagues discovered a method of isolating and studying C-reactive protein by using the protein's affinity for phosphoryl choline that has been immobilized to a porous solid chromatography support.Highlights:
Ligand – p-aminophenyl phosphoryl choline
Support – crosslinked 6% beaded agarose
Binding capacity – greater than 3mg of human C-reactive protein per milliliter of settled resin
Format – 50% resin slurry in 0.02% sodium azide
Binding Condition – Tris or borate-buffered saline (pH 8 to 8.5) with 1 to 2mM calcium chloride
Elution Condition – Tris or borate-buffered saline (pH 8 to 8.5) with 2mM EDTA
Reusable – resin can be regenerated and reused several times
Recommended for:
Purify c-reactive protein (CRP); Purify anti-p-Aminophenyl phosphoryl choline antibodies

Specifications
Upon receipt store at 4°C. | |
Pierce™ | |
C-reactive Protein | |
p-Amino Phosphoryl Choline | |
Affinity, Agarose Resin |
Liquid | |
5 mL | |
Agarose | |
Bottle |
Safety and Handling
- EZ-Link Micro Sulfo-NHS-Biotinylation Kit
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H312-Harmful in contact with skin.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P271-Use only outdoors or in a well-ventilated area.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulfo-NHS-Biotin, Sodium chloride, Sodium phosphate dibasic
Certificates
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For Research Use Only. Not for use in diagnostic procedures.