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Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis
Ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.
96.00€ - 720.00€
Specifications
Product Type | Cell Lysis Enzyme |
---|---|
Content And Storage | Store in a cool, dry, well-ventilated area, protected from direct sunlight. |
Form | Liquid |
Product Line | Pierce™ |
Reagent Type | Enzyme for Cell Lysis |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
12146490
|
Thermo Scientific™
88700 |
5 kU |
96.00€
5ku |
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12391963
|
Thermo Scientific™
88702 |
100 kU |
720.00€
100ku |
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12156490
|
Thermo Scientific™
88701 |
25 kU |
336.00€
25ku |
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Description
Thermo Scientific Pierce Universal Nuclease for Cell Lysis is an endonuclease that is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.
Features of Universal Nuclease for Cell Lysis include:
- Broad spectrum—degrades all forms of DNA and RNA
- Highest-quality enzyme—nuclease is ≥99% pure, as tested by SDS-PAGE
- Robust activity—100-fold greater specific activity than DNase I
- Versatile—can be used with a wide variety of cell lysis reagents
Pierce Universal Nuclease for Cell Lysis is a genetically engineered endonuclease from Serratia marcescens. The enzyme is produced and purified from E. coli and consists of two identical 30-kDa subunits with two critical disulfide bonds. This indiscriminate endonuclease degrades single-stranded, double-stranded, linear, and circular DNA and RNA and is effective over a wide range of temperatures and pH.
Pierce Universal Nuclease for Cell Lysis has high specific activity (100-fold greater than DNase I) and increased thermal stability compared to other nucleases. It is ≥99% pure enzyme, free of any measurable protease activity, and supplied at 250U/μL. It is identical in performance to Benzonase™ Nuclease (EMD Merck).
Applications
- Use with B-PER, Y-PER, or other commercial or homebrew cell lysis reagents and/or mechanical disruption to reduce viscosity in protein extracts
- Use to remove DNA and RNA from recombinant protein preparations prior to downstream processing
Pierce Universal Nuclease for Cell Lysis is commonly used to reduce the viscosity of bacterial and mammalian protein extracts for downstream application by removing the nucleic acids from protein preparations. The enzyme completely digests nucleic acids to oligonucleotides that are less than five bases long. It helps to improve the separation of the lysate pellet from the supernatant, enhances filtration of the treated lysate, improves chromatography processing time, and increases the overall protein yield. It has also been shown to improve the compatibility of protein extracts for 2D gel electrophoresis.
One unit corresponds to the amount of enzyme required to produce a change of 1.0 in the absorbance at 260 nm of sonicated herring DNA over 30 minutes at 37°C, as determined using standard nuclease from the Merck™ Serratia marcescens volumetric activity assay. Add 25 U, or 0.1 µL, of Pierce Universal Nuclease for Cell Lysis to 1 mL of cell lysate and incubate at room temperature for 10–15 min.
Specifications
Cell Lysis Enzyme | |
Liquid | |
Enzyme for Cell Lysis |
Store in a cool, dry, well-ventilated area, protected from direct sunlight. | |
Pierce™ | |
Nuclease |
For Research Use Only. Not for use in diagnostic procedures.
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