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Macherey-Nagel™ Protein Quantification Assay
NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein
36.50€ - 104.00€
Specifications
| Concentration | 10 to 20000ng/μL |
|---|---|
| Detectable Analytes | Protein |
| Immunoassay Kit Format | Buffer and Reagent Set |
| Product Type | Protein Quantification Assay |
| Sample Type | Protein |
Description
NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein.Superior selectivity - purify your RNA fractionated by size
Isolation of small RNA only (18 to 200 bases)Isolation of small (18 to 200 bases) and large (>200 bases) RNA in two fractionsIsolation of total RNA (small and large in one fraction)
Additional isolation of total protein
Denatured protein ready-to-use for analysis in SDS-PAGE and Western blot.
Excellent RNA recovery - even without phenol/chloroform
Lysis with chaotropic saltSpin column based procedure
Convenient handling - complete equipment provided
NucleoSpin filters - efficient sample homogenisationNucleoSpin protein removal columns - highest purity of small RNArDNase - highly efficient on-column digestion of genomic DNA at RT
- Silica membrane technology
- Format: minispin columns
- Sample size: <107 cultured cells, <30mg human/tissue; <50mg plant tissue, <150μL reaction mixture
- Fragment size, small RNA: 18 to 200 bases
- Fragment size, large RNA: >200 bases
- Typical yield: 10μg small RNA; 95μg large RNA from 107 HeLa cells
- Binding capacity: 200μg
- Elution volume, 30μL to 100μL
- Preparation time: <45min (6 preps human/animal tissue, small and large RNA); <35min (6 preps human/animal tissue, small RNA only)
- Small RNA (18 to 200 bases)
- Large RNA (>200 bases)
- Total RNA (small and large RNA in one fraction), and isolate your total protein fraction For small and large RNA and total RNA purification, and isolation of total protein fraction.
Specifications
| 10 to 20000ng/μL | |
| Buffer and Reagent Set | |
| Protein | |
| Small and large RNA and total RNA purification | |
| 1 Each | |
| 40 min. |
| Protein | |
| Protein Quantification Assay | |
| 107 | |
| Chaotropic salt | |
| 40 min. |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Substances and mixtures corrosive to metals Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H290-May be corrosive to metals.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
Supplemental information
- MIXTURE LIST-Contains : hydrochloric acid
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