DiscoverX Ready-to-Assay™ EP3 Prostanoid Receptor Frozen Cells

Description

The Ready-to-Assay™ EP3 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTGER3 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER3 , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the cells in a convenient thaw-and-use format.