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R&D Systems™ Recombinant Mouse Plasma Kallikrein/KLKB1 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity
Brand: R&D Systems™ 2498-SE-010
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Description
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002 ). The specific activity is >300 pmol/min/μg, as measured under the described conditions.Specifications
| NP_032481 | |
| Lyophilized from a 0.2 μm filtered solution in MES and NaCl. | |
| M.W. (Observed): 80 kDa, reducing conditions; M.W. (theoretical): 70 kDa | |
| 10 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
| <1.0 EU / 1 μg of the protein by the LAL method. | |
| Unconjugated | |
| Reconstitute at 500 μg/mL in sterile 25 mM MES and 150 mM NaCl, pH 5.5. | |
| Plasma Kallikrein/KLKB1 |
| Enzyme Activity | |
| 3818 | |
| Plasma Kallikrein/KLKB1 | |
| Mouse myeloma cell line, NS0-derived mouse Plasma Kallikrein/KLKB1 protein Gly20-Ala638, with a C-terminal 6-His tag | |
| Pro form | |
| Klkb1 | |
| Recombinant | |
| >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
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