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  6. Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Base Kit
  7. 11521306

Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Base Kit

TaqMan™ Protein Assays Core Reagents Base Kit

Brand:  Applied Biosystems™ 4405460

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Product Code. 11521306

  • 534.00€ / 100 reactions

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Description

Description

TaqMan™ Protein Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan™ protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan™ probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system.
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Specifications

Specifications

7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, AB 7900 HT 384-Well System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Proximity Ligation Assay
Primer-Probe Detection
Reagents for Protein Expression
Cells
TaqMan™
Fast
Not Target-Specific
TaqMan™
The TaqMan™ Protein Assays Core Reagents Base Kit contains:

DNA Ligase, 500X (1 × 10 μL)
Ligase Dilution Buffer, 1X (2 × 1.5 mL)
Ligation Reaction Buffer, 20X (1 × 0.7 mL)
Phosphate Buffered Saline (PBS), 1X, pH 7.4 (1 × 1 mL)
Protease, 100X (1 × 10 μL)
Universal PCR Assay, 20X (1 × 120 μL)
Store all components at -15 to -25°C.
FAM
100 Reactions
100 reactions
qPCR Protein Quantification
Wet Ice
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Videos
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Documents

Documents

Product Information

Manuals

Quick Reference Card: TaqMan® Protein Assays (English ) Version: 16 Feb 2011 File size: 661.6 kB
Manual: Real-Time PCR Systems TaqMan® Protein Assays Chemistry Guide (English ) Version: 13 Apr 2011 File size: 3.3 MB
Quick Reference Card: TaqMan Protein Assays Version: 4449281 Rev B File size: 661.6 kB
Protocol: TaqMan® Protein Assays Sample Prep and Assay (English ) Version: 16 Feb 2011 File size: 2.6 MB
Manual: ProteinAssist™ Software Getting Started Guide (English ) Version: 08 Nov 2010 File size: 6.7 MB

Specification Sheets

Brochures & Specifications: TaqMan® Protein Assays Brochure File size: 2.1 MB
Brochures & Specifications: TaqMan Protein Assays Product Overview File size: 346.7 kB
Brochures & Specifications: TaqMan Protein Expression Assays File size: 957.6 kB

Scientific Resources

Posters

Novel Protein Expression Assays using qPCR for the Detection and Relative Quantification of Protein Markers in Stem Cells File size: 230.4 kB
An integrated qPCR approach for monitoring gene and protein expression in human pluripotent & differentiated cells File size: 170.9 kB
MicroRNA dynamics in early neuronal differentiation with correlation to protein output and cell biology File size: 753.4 kB
Detection of clinically relevant proteins in human testicular germ cell tumors File size: 243.8 kB

Application Notes

Application Notes & Tutorials: Designing Assays for the TaqMan® Protein Assay File size: 312.8 kB
Application Notes & Tutorials: Novel Protein Assays Using TaqMan® Chemistry for the Detection and File size: 462.2 kB
Certificates
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Special Offers

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