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Bioluminescence Assays and Reagents

Bioluminescence Assays and Reagents

Luciferase vectors, cell lysis buffers, signal enhancers, transfection reagents, assay kits, and other associated products for optimal luciferase reporter assay performance.
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1 – 15 of 45 results
1

Thermo Scientific™ TurboFect™ Transfection Reagent

Deliver DNA plasmids and expression vectors into eukaryotic cells with this highly efficient, easy-to-use, non-immunogenic transfection reagent.

Promotions are available
EDGE
Type Transfection Reagent
2

Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters

Promotions are available
Substrate D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Detection Method Bioluminescence
3

Thermo Scientific™ Pierce™ Cypridina Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in mammalian cell culture media and whole cell lysate with this highly sensitive flash-based luciferase kit.

Promotions are available
Substrate Vargulin (Cypridina Luciferin)
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Cypridina Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce™
Detection Method Bioluminescence
4

Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit

Perform firefly luciferase assays in high-throughput screening applications.

Promotions are available
Substrate D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase, Cypridina Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Detection Method Bioluminescence
5

Thermo Scientific™ Pierce™ Gaussia Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Gaussia luciferase reporters

Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Detection Method Bioluminescence
Promoter EF-1α
Substrate Coelenterazine
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Gaussia Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce™
6

Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in mammalian culture media or whole cell lysates with this highly sensitive flash-based Gaussia luciferase kit.

Promotions are available
Substrate Coelenterazine
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Gaussia Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce™
Detection Method Bioluminescence
7

Thermo Scientific™ Pierce™ Renilla Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life approx. 3 hour) in the presence of Renilla luciferase reporters.

Substrate Coelenterazine
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Green Renilla Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce™
Detection Method Bioluminescence
8

Thermo Scientific™ pMCS-Gaussia-Dura Luc Vector for Luciferase Assays

Drive mutated Gaussia luciferase expression with vectors containing a multiple cloning site (MCS) or control promoter (CMV or TK) for extended signal output.

9

Thermo Scientific™ pMCS minP-Tluc16 Hygro Vector for Luciferase Assays

Insert promoter sequences for genes of interest into Thermo Scientific™ Tluc16 Vectors to measure gene regulation using the intracellular TurboLuc™ 16 (Tluc16) luciferase reporter in luciferase assays.

10

Thermo Scientific™ pCRE Tluc16-DD Vector for Luciferase Assays

Insert promoter sequences for genes of interest into Thermo Scientific™ Tluc16 Vectors to measure gene regulation using the intracellular TurboLuc™ 16 (Tluc16) luciferase reporter in luciferase assays.

11

Thermo Scientific™ pMCS-Cypridina Luc Vector for Luciferase Assays

Drive Cypridina luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).

12

Thermo Scientific™ Pierce™ Luciferase Cell Lysis Buffer (2X)

Lyse adherent cells or pelleted cell suspensions for analysis of promoter expression in luciferase assays with this optimized protein extraction reagent.

13

Thermo Scientific™ TurboLuc™ Luciferase One-Step Glow Assay Kit

Measure TurboLuc™16 (Tluc16) luciferase activity in mammalian cells with a single reagent-addition step that is for high-throughput screening (HTS) applications using the Thermo Scientific™ TurboLuc™ Luciferase One-Step Glow Assay Kit.

Substrate Coelenterazine
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, TurboLuc™18 Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Detection Method Bioluminescence
14

Thermo Scientific™ pMCS minP-Tluc16 Vector for Luciferase Assays

Insert promoter sequences for genes of interest into Thermo Scientific™ Tluc16 Vectors to measure gene regulation using the intracellular TurboLuc™ 16 (Tluc16) luciferase reporter in luciferase assays.

15

Thermo Scientific™ Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit

Simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

Substrate Vargulin (Cypridina Luciferin), D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Cypridina Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce™
Detection Method Bioluminescence