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Reverse Transcription Products

Reverse Transcription Products

A variety of kits, reagents, and mixes suitable for reverse transcription processes. Products include one-step RT-PCR reagent, cDNA synthesis kits and master mixes, and standalone reverse transcriptases.
cDNA Synthesis Kits and Master Mixes (22)
Reverse Transcriptases (12)
One-Step Reverse Transcription PCR Reagents (9)

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1 – 15 of 17 results
1

Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase RevertAid H Minus
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C to 45°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
2

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Shipping Condition Dry Ice
Polymerase Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
GC-Rich PCR Performance Medium
Content And Storage Store at –15 to –25°C.
Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Format Single-Tube
Form Liquid
Reaction Speed Fast or Standard
Hot Start Antibody-based
Shelf Life Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
For Use With (Application) Virus Detection,Pathogen Detection,Real Time PCR (qPCR),Gene Expression
PCR Method 1-step RT-qPCR,Multiplex qPCR
Product Line TaqPath™
Detection Method Primer-probe
3

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Promotions are available
Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
For Use With (Application) RT-PCR
Format Kit
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
4

Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.

Promotions are available
EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima H Minus
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 20 kb
Reaction Format Separate components
Reaction Speed 15 to 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C to 55°C
Technique Reverse Transcription
Starting Material RNA
5

Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.

Promotions are available
EDGE
Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
6

Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR

The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima
Final Product Type First-Strand cDNA
For Use With (Application) Real Time PCR (qPCR)
Format Kit
Size (Final Product) Up to 20 kb
Reaction Format Separate components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 50°C to 55°C
Technique Reverse Transcription
Starting Material RNA
7

Thermo Scientific™ RevertAid RT Reverse Transcription Kit

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.

EDGE
8

Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)

Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.

Promotions are available
EDGE
Concentration 200 U/μL
9

Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.

Promotions are available
EDGE
Shipping Condition Dry Ice
Content And Storage

• RevertAid H Minus Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.
Reverse Transcriptase RevertAid H Minus
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C to 45°C
Technique Reverse Transcription
Starting Material RNA
10

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix

Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix, available with or without dsDNase, provides all cDNA synthesis reaction components in a convenient one-tube master mix.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima H Minus
Final Product Type First-Strand cDNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Format Master Mix
Size (Final Product) Up to 20 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
11

Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit

Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA.

Promotions are available
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Format Kit
Reverse Transcriptase Maxima H Minus
Size (Final Product) Up to 20 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C to 55°C
Technique Reverse Transcription
Starting Material RNA
12

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Content And Storage Store at –15 to –25°C.
Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form Liquid
Concentration 4X
For Use With (Application) Pathogen Detection,Virus Detection,Real Time PCR (qPCR),Gene Expression
PCR Method 1-step RT-qPCR,Multiplex qPCR
Detection Method Primer-probe
Polymerase Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format Single-Tube
Passive Reference Dye ROX™
Reaction Speed Fast or Standard
Hot Start Antibody-based
Shelf Life Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line TaqPath™
13

Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.

GC-Rich PCR Performance High
Format 1-step RT-PCR Kit
Reverse Transcriptase SuperScript IV
Size (Final Product) Up to 13 kb
Reaction Format Master Mix
Reagent Type Reverse Transcription
Hot Start Built-in Hot Start
Optimal Reaction Temperature 50°C to 65°C
Technique 1-step RT-PCR
Starting Material RNA
PCR Method 1-step RT-PCR
Multiplex Capability Yes
Detection Method Gel Electrophoresis
14

Thermo Scientific™ Verso cDNA Synthesis Kit

The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool.

EDGE
Shipping Condition Wet or Dry Ice
Format Kit
Reverse Transcriptase Verso
Size (Final Product) Up to 11 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
15

Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit

The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.

Reverse Transcriptase Maxima H Minus
Reaction Format Separate Components
Final Product Type Double Stranded cDNA
Product Type cDNA Synthesis Kit
Sample Type RNA
For Use With (Application) Cloning
Product Line Maxima