Test
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Product Type | T4 DNA Ligase |
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Enzyme | DNA Ligase |
Compatible Buffer | 10X T4 DNA Ligase Buffer |
Concentration | 5 U/μL |
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
Product Type | Exonuclease I |
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Enzyme | Exonuclease |
Concentration | 20 U/μL |
Digest single-stranded and double-stranded DNA and RNA with Thermo Scientific™ Micrococcal Nuclease (S7 Nuclease), an endo-exonuclease.
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.
Content And Storage | Uracil-DNA glycolase (1 U/μL) and 10X Reaction Buffer. Store at -20°C. |
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Product Type | Uracil DNA Glycosylase |
Enzyme | Uracil-DNA Glycosylase |
Compatible Buffer | 10X Reaction Buffer |
Concentration | 1 U/μL |
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.
Product Type | RNase T1 |
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Enzyme | RNase |
Concentration | 1000 U/μL |
Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations.
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
Product Type | RNase H |
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Enzyme | RNase |
Compatible Buffer | 10X Reaction Buffer |
Concentration | 5 U/μL |
Catalyze the ATP-dependent formation of phosphodiester bonds between 5'-P and 3'-OH termini of oligonucleotides, single-stranded RNA and DNA.
Get the RNA degradation activity of both RNase A and RNase T1 with Thermo Scientific™ RNase A/T1 Mix.
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Form | Liquid |
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Product Type | Proteinase K |
RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.
Product Type | RNase Inhibitor |
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Compatible Buffer | Storage Buffer |
Concentration | 40 U/μL |
Product Line | RiboLock™ |
Digest double-stranded DNA or DNA-RNA hybrids with Exonuclease III is a 3'–>5' exonuclease.