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Thermo Scientific™ HisPur™ Cobalt Superflow Agarose
Perform high-purity, large-scale purification of polyhistidine-tagged proteins with this tetradentate-chelating Superflow 6 support charged with divalent cobalt.
Brand: Thermo Scientific™ 25228
100.11 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
Perform high-purity, large-scale purification of polyhistidine-tagged proteins with Thermo Scientific™ HisPur Cobalt Superflow Agarose, a tetradentate-chelating Superflow 6 support charged with divalent cobalt.
Additional Information and Online Ordering
HisPur Cobalt Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to nickel and other ligands commonly used for immobilized metal affinity chromatography (IMAC), cobalt provides greater specificity for His-tagged protein purification. The specific binding of HisPur Cobalt Superflow Agarose results in purer elution fractions, reducing the need for extra polishing steps.Highlights:
Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour with minimal leaching of cobalt due to a tetradentate chelator
High yields – binds 20mg of 6xHis-GFP per mL of resin at a linear flow rate of 150cm/hour, can bind greater than 30mg per mL of resin depending on flow rate
High purity – specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins
Compatible – maintains function after exposure to a wide variety of chemicals and pH values
Cost effective – resin is stable through multiple cycles of cleaning and reuse
Recommended for:
Large scale FPLC purification of polyhistidine tagged proteins
Specifications
| Store at 4°C | |
| HisPur™ | |
| His-Tagged Protein | |
| Metal Chelate, Divalent Cobalt | |
| Affinity, Agarose Resin |
| Liquid Suspension | |
| 10 mL | |
| Agarose | |
| Bottle |
For Research Use Only. Not for use in diagnostic procedures.