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Thermo Scientific™ HisPur™ Cobalt Superflow Agarose
Perform high-purity, large-scale purification of polyhistidine-tagged proteins with this tetradentate-chelating Superflow 6 support charged with divalent cobalt.
141.00€ - 8030.00€
Specifications
| Content And Storage | Store at 4°C |
|---|---|
| Form | Liquid Suspension |
| Product Line | HisPur™ |
| Target | His-Tagged Protein |
| Type | Agarose |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
13474179
|
Thermo Scientific™
25228 |
10 mL |
141.00€
10mL |
Estimated Shipment: 06-12-2024
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|
13484179
|
Thermo Scientific™
25231 |
1 L |
8030.00€
1 L |
Estimated Shipment: 03-12-2024
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|
13414189
|
Thermo Scientific™
25230 |
250 mL |
2715.00€
250mL |
Estimated Shipment: 03-12-2024
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|
13404189
|
Thermo Scientific™
25229 |
50 mL |
622.00€
50mL |
Estimated Shipment: 12-12-2024
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Description
Perform high-purity, large-scale purification of polyhistidine-tagged proteins with Thermo Scientific™ HisPur Cobalt Superflow Agarose, a tetradentate-chelating Superflow 6 support charged with divalent cobalt.
Additional Information and Online Ordering
HisPur Cobalt Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to nickel and other ligands commonly used for immobilized metal affinity chromatography (IMAC), cobalt provides greater specificity for His-tagged protein purification. The specific binding of HisPur Cobalt Superflow Agarose results in purer elution fractions, reducing the need for extra polishing steps.Highlights:
Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour with minimal leaching of cobalt due to a tetradentate chelator
High yields – binds 20mg of 6xHis-GFP per mL of resin at a linear flow rate of 150cm/hour, can bind greater than 30mg per mL of resin depending on flow rate
High purity – specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins
Compatible – maintains function after exposure to a wide variety of chemicals and pH values
Cost effective – resin is stable through multiple cycles of cleaning and reuse
Recommended for:
Large scale FPLC purification of polyhistidine tagged proteins
Specifications
| Store at 4°C | |
| HisPur™ | |
| Agarose | |
| Bottle |
| Liquid Suspension | |
| His-Tagged Protein | |
| Metal Chelate, Divalent Cobalt | |
| Affinity, Agarose Resin |
For Research Use Only. Not for use in diagnostic procedures.