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Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads

Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.

Brand:  Thermo Scientific™ 88832

441.66 EUR valid until 2024-11-30
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Product Code. 13484289

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Description

Description

Perform affinity purification of His-tagged fusion proteins in manual or automated formats with Thermo Scientific™ HisPur Ni-NTA Magnetic Beads, high capacity nickel-IMAC beads.

Additional Information and Online Ordering

The blocked magnetic bead surface is derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) beads provide high binding capacity with very low background. The HisPur Ni–NTA Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific™ KingFisher Instruments for high–throughput needs.

Highlights:

  • High capacity – equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers

  • Low nonspecific binding – the bead surface is pre-blocked and the protocol provides optimized buffers for purification

  • Fast – protocol is completed in 1 hour

  • Scalable – process microliter to milliliter sample volumes

  • Versatile – purify proteins using native or denaturing conditions

  • Reagent compatible – can be used with common cell lysis reagents and a variety of buffer additives

  • Multiple formats – protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific™ KingFisher Instruments)

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Store at 4°C
10 mL
His
Nickel-NTA
HisPur™
Recombinant
Magnetic Affinity Bead
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Safety and Handling

Safety and Handling

Product Identifier
  • HisPur Ni-NTA Magnetic Beads

Signal Word
  • Danger

Hazard Category
  • Carcinogenicity Category 2
  • Serious eye damage/eye irritation Category 2
  • Reproductive toxicity Category 1
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1

Hazard Statement
  • H228-Flammable solid.
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
  • H350-May cause cancer.
  • H360-May damage fertility or the unborn child.

Precautionary Statement
  • P202-Do not handle until all safety precautions have been read and understood.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : ethanol, sulphuric acid

SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.