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Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads
Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.
237.00€ - 866.00€
Specifications
| Content And Storage | Store at 4°C |
|---|---|
| Product Line | HisPur™ |
| Protein Form | Recombinant |
| Target | His |
| Type | Magnetic Affinity Bead |
Description
Perform affinity purification of His-tagged fusion proteins in manual or automated formats with Thermo Scientific™ HisPur Ni-NTA Magnetic Beads, high capacity nickel-IMAC beads.
Additional Information and Online Ordering
The blocked magnetic bead surface is derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) beads provide high binding capacity with very low background. The HisPur Ni–NTA Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific™ KingFisher Instruments for high–throughput needs.Highlights:
High capacity – equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers
Low nonspecific binding – the bead surface is pre-blocked and the protocol provides optimized buffers for purification
Fast – protocol is completed in 1 hour
Scalable – process microliter to milliliter sample volumes
Versatile – purify proteins using native or denaturing conditions
Reagent compatible – can be used with common cell lysis reagents and a variety of buffer additives
Multiple formats – protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific™ KingFisher Instruments)
Specifications
| Store at 4°C | |
| Recombinant | |
| Magnetic Affinity Bead |
| HisPur™ | |
| His | |
| Nickel-NTA |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- HisPur Ni-NTA Magnetic Beads
Signal Word
- Danger
Hazard Category
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 2
- Reproductive toxicity Category 1
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
Hazard Statement
- H228-Flammable solid.
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H319-Causes serious eye irritation.
- H350-May cause cancer.
- H360-May damage fertility or the unborn child.
Precautionary Statement
- P202-Do not handle until all safety precautions have been read and understood.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol, sulphuric acid
For Research Use Only. Not for use in diagnostic procedures.