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IBA Lifesciences StrepMAB-Immo Conjugate

StrepMAB-Immo conjugates are monoclonal antibodies with different fluorescent conjugates to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins
Brand: IBA Lifesciences 2-1570-050
Description
StrepMAB-Immo fluorescenct conjugates are murine, high-affinity monoclonal antibodies, which are especially suited for detection of Strep-tag™II and Twin-Strep-tag™ fusion proteins in applications like ELISA, FACS, immunohistochemistry, and immunocytochemistry.The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag™II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein).
Proteins containing Strep-tag™II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far, the Twin-Strep-tag™ always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). StrepMAB-Immo is available as conjugate with different fluorescent dyes (DY-488, DY-549, DY-649).
The absorption/emission maxima of the fluorescent dyes are:
• DY-488: 493/518 nm
• DY-549: 550-560/575 nm
• DY-649: 655/676 nm
Please note: For detection of Strep-tag™II or Twin-Strep-tag™ fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin™ is recommended.
Specifications
0.10 mL | |
Solution in PBS; 0.01 % NaN3; 1 mg/ mL BSA | |
50 ÎĽg | |
Strep-tag™II and Twin-Strep-tag™ | |
DY-649 |
0.5 mg/mL | |
Undiluted: 2°C to 8°C, protect from light; Diluted: -25°C to -15°C | |
ELISA, FACS, immunofluorescence | |
Conjugate |
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