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Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Base Kit

TaqMan™ Protein Assays Core Reagents Base Kit

Brand: Applied Biosystems™ 4448592

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Product Code. 12047799

1582.00€ / 500 reactions

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Description

TaqMan™ Protein Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan™ protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan™ probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system.

Specifications

For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOnePlus™, Fast Mode
For Use With (Application)	Real Time PCR (qPCR), Stem Cell Analysis
Detection Method	Primer-Probe Detection
Product Type	Protein Assays Core Reagents Base Kit
Sample Type	Tissue, Cells
No. of Reactions	500
Polymerase	AmpliTaq™ Fast DNA Polymerase
PCR Method	qPCR Protein Quantification
Shipping Condition	Wet Ice

Product Line	TaqMan™
Content And Storage	The TaqMan™ Protein Assays Core Reagents Base Kit contains: DNA Ligase, 500X (1 × 50 μL) Ligase Dilution Buffer, 1X (1 × 15 mL) Ligation Reaction Buffer, 20X (1 × 4 mL) Phosphate Buffered Saline (PBS), 1X, pH 7.4 (1 × 5 mL) Protease, 100X (1 × 50 μL) Universal PCR Assay, 20X (1 × 600 μL) Store all components at -15 to -25°C.
Label or Dye	FAM
Quantity	500 reactions
Hot Start	No
Passive Reference Dye	ROX (Pre-mixed)
Primer Probe Compatibility	TaqMan™
Reaction Speed	Fast
Target Specificity	Not Target-Specific

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Documents

Product Information

Manuals

Getting Started Guide - ProteinAssist Software - Data analysis software for TaqMan Protein Assays Version: 4457491 Rev B File size: 6.7 MB

Quick Reference Card: TaqMan® Protein Assays (English ) Version: 16 Feb 2011 File size: 661.6 kB

Manual: Real-Time PCR Systems TaqMan® Protein Assays Chemistry Guide (English ) Version: 13 Apr 2011 File size: 3.3 MB

Quick Reference Card: TaqMan Protein Assays Version: 4449281 Rev B File size: 661.6 kB

Protocol: TaqMan® Protein Assays Sample Prep and Assay (English ) Version: 16 Feb 2011 File size: 2.6 MB

TaqMan® Protein Assays Core Reagent Kit, 500 Insert Version: 4448597 Jun 2010 File size: 123.7 kB

Manual: ProteinAssist™ Software Getting Started Guide (English ) Version: 08 Nov 2010 File size: 6.7 MB

Specification Sheets

Brochures & Specifications: TaqMan® Protein Assays Brochure File size: 2.1 MB

Brochures & Specifications: TaqMan Protein Assays Product Overview File size: 346.7 kB

Scientific Resources

Posters

An integrated qPCR approach for monitoring gene and protein expression in human pluripotent & differentiated cells File size: 170.9 kB

MicroRNA dynamics in early neuronal differentiation with correlation to protein output and cell biology File size: 753.4 kB

Detection of clinically relevant proteins in human testicular germ cell tumors File size: 243.8 kB

Application Notes

Application Notes & Tutorials: Designing Assays for the TaqMan® Protein Assay File size: 312.8 kB

Application Notes & Tutorials: Novel Protein Assays Using TaqMan® Chemistry for the Detection and File size: 462.2 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2802765	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4448592
Lot Number2847541	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number4448592
Lot Number2850569	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number4448592
Lot Number2858151	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number4448592
Lot Number2862821	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number4448592

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