All Primary Antibodies
All Primary Antibodies
Primary antibodies, immunoglobulins that bind to specific proteins or other biomolecules, are used in many research applications and protocols to detect targets of interest. They are developed using different animal hosts, including mouse, rat, rabbit, goat, sheep, and many others.
Monoclonal and Polyclonal Antibodies
One type of primary antibodies, monoclonal antibodies, provides high reproducibility and low cross-reactivity and background noise. Another type, polyclonal antibodies, often costs less and provides greater affinity and quicker binding. Both are produced using plasma B cells, but the former uses the same clone and the latter uses different clones. Monoclonal antibodies require hybridoma cell lines, and polyclonal antibodies do not.
There are also recombinant monoclonal antibodies with similar benefits, like high affinity, scalability, and specificity. They are produced using in vitro cloning of plasma B cells and expression hosts.
Conjugated Primary Antibodies
Antibodies can be labeled with various fluorophores or detection agents or used without labels. Labeled primary antibodies, also known as conjugated primary antibodies, help researchers simplify and streamline their applications. They are coupled with common enzymes and dyes such as Alexa Fluor and often used in protein and cell analysis.
Applications
Antibodies for life sciences applications are used in flow cytometry, western blotting, ELISA, immunohistochemistry, and immunocytochemistry. Secondary antibodies can be added to support the detection and purification of certain antigens. They bind to the primary antibody, which binds to the antigen of interest. Finding the right combination of antibodies can result in greater antigen specificity and a strong, detectable signal.
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| Antigen | Fc Block |
|---|---|
| Regulatory Status | RUO |
| Content And Storage | Store undiluted at 4°C |
| Target Species | Human,Rhesus Monkey |
| Conjugate | Unconjugated |
| Applications | Flow Cytometry |
| Form | Purified |
| Classification | Monoclonal |
| Primary or Secondary | Primary |
| Concentration | 0.5mg/mL |
| Clone | 3070 |
| Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
|---|---|
| Target Species | Multi-species |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | Western Blot |
| Form | Lyophilized |
| Isotype | IgG2b |
| Antigen | Luciferase |
| Purification Method | Protein A or G purified from hybridoma culture supernatant |
| Dilution | Western Blot 2 ÎĽg/mL |
| Gene Alias | ec 1.13.12.7, Fluc, luciferin 4 monooxygenase |
| Formulation | Lyophilized from a 0.2 ÎĽm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 ÎĽm filtered solution in PBS. |
| Immunogen | Synthetic peptide of NanoLuc™ (Nluc) Luciferase |
| Classification | Monoclonal |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. |
| Primary or Secondary | Primary |
| Test Specificity | Detects NanoLuc™ (Nluc) Luciferase in direct ELISAs. Detects NanoLuc™ (Nluc) Luciferase and Large BiT in Western blots. |
| Clone | 965808 |
Abnova Fibronectin, Sheep, Polyclonal Antibody, Abnova™
Sheep polyclonal antibody raised against native Fibronectin.
R&D Systems Human TNF RII/TNFRSF1B Antibody, R&D Systems™
Mouse Monoclonal Antibody has been used in 9 publications
| Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | Western Blot,Neutralization,ELISA |
| Isotype | IgG1 |
| Gene Accession No. | P20333 |
| Antigen | TNF RII/TNFRSF1B |
| Gene Symbols | TNFRSF1B |
| Regulatory Status | RUO |
| Purification Method | Protein A or G purified from hybridoma culture supernatant |
| Dilution | Western Blot 1 ug/mL, Neutralization 0.5-1.5 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL |
| Gene Alias | CD120b, CD120b antigen, Etanercept, p75 TNF receptor, p75TBPII, p75TNFR, soluble TNFR1B variant 1, TNFBRp80 TNF-alpha receptor, TNF-R2, TNFR2TNFR1B, TNF-R75, TNFR80, TNFRII, TNF-RII, TNF-R-II, TNFR-II, TNFRSF1B, tumor necrosis factor beta receptor, tumor necrosis factor binding protein 2, Tumor necrosis factor receptor 2, tumor necrosis factor receptor superfamily member 1B, tumor necrosis factor receptor superfamily, member 1B, Tumor necrosis factor receptor type II |
| Gene ID (Entrez) | 7133 |
| Formulation | Lyophilized from a 0.2 ÎĽm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 ÎĽm filtered solution in PBS. with No Preservative |
| Immunogen | E. coli-derived recombinant human TNF RII/TNFRSF1B Leu23-Asp257 Accession # P20333 |
| Classification | Monoclonal |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. |
| Primary or Secondary | Primary |
| Test Specificity | Detects human TNF RII in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) TNF RI, recombinant mouse (rm) TNF RI, rmTNF RII, rmTNF-alpha , rhTNF-alpha , or rhTNF-beta . In Western blots, this antibody does not cross-react with rhTNF RI, rmTNF RI, or rmTNF RII. |
| Clone | 22210 |
R&D Systems Mouse Podocalyxin Antibody, R&D Systems™
Goat Polyclonal Antibody has been used in 32 publications
Abnova Helicobacter pylori Mouse anti-Helicobacter pylori, Clone: J353, Abnova™
Mouse Monoclonal Antibody
Abnova Helicobacter pylori Mouse anti-Helicobacter pylori, Clone: J354, Abnova™
Mouse Monoclonal Antibody
| Content And Storage | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
|---|---|
| Target Species | Mouse |
| Host Species | Rat |
| Conjugate | PE |
| Isotype | IgG2a Îş |
| Concentration | 0.2mg/mL |
| Antigen | IL-33R (ST2) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Gene Alias | Il1rl1, ST2, ST2L, IL-33R alpha, IL-33Rα, IL33RA, Ly84, DER4, Fit-1 |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Immunogen | Mouse IL-33R Recombinant Protein |
| Classification | Monoclonal |
| Primary or Secondary | Primary |
| Clone | U29-93 |
| Antigen | CD21 |
|---|---|
| Regulatory Status | RUO |
| Content And Storage | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Purification Method | Affinity Purified |
| Description | CR2; Complement receptor type 2; C3DR; EBV-R; Epstein-Barr virus receptor |
| Target Species | Baboon,Cynomolgus,Human,Pig,Rhesus Monkey |
| Host Species | Mouse |
| Conjugate | PE |
| Applications | Flow Cytometry |
| Formulation | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
| Classification | Monoclonal |
| Isotype | IgG1 Îş |
| Primary or Secondary | Primary |
BD Biosciences TCR β Chain Hamster anti-Mouse, Brilliant Violet 421, Clone: H57-597, BD
Hamster Monoclonal Antibody